The cloning vectors must possess the following general characteristics: It is selected based upon the size and the kind of DNA segment to be cloned. Most of the cloning vectors are genetically engineered. It can be extracted from a virus, plasmid or cells of a higher organism. For easy incorporation into the host machinery, a vector should itself be small in size and be able to integrate large size of the insert.Ī cloning vector is also a fragment of DNA which is capable of self-replication and stable maintenance inside the host organism.This selectable marker can be an antibiotic resistance gene. A vector should always harbour a selectable marker to screen the recombinant organism.It must possess a compatible restriction site for insertion of DNA molecule.
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